Cell Culture:Article Title: TCP Doped with Metal Ions Reinforced with Tetragonal and Cubic Zirconia.
Article Snippet: Thus, a cell line of hOB (Cell Applications, Inc., San Diego, CA, USA), t-flasks of 75 cm3 cell culture (Orange Scientific, Braine-l’Alleud, Belgium), Dulbecco’s Modified Eagle Medium/Nutrient F-12 (DMEM-F12, Sigma-Aldrich, Sintra, Portugal), sodium bicarbonate (NaHCO3, Labchem, Laborspirit, Santo Antão do Tojal, Portugal, CAS: 144-55-8), double deionized water (ultrapure water, obtained using an ultrapure water purification system Milli-Q Advantage A10, filtered at 0.22 μm and 18.2 MΩ cm at 25 ◦C), bovine fetal serum (FBS, Biochrom AG, Berlin, Germany, CAS: 9014- 81-7), trypsin (Sigma-Aldrich, Sintra, Portugal, CAS: 9002-07-7), resazurin (Sigma-Aldrich, Sintra, Portugal, CAS: 62758-13-8), spectrofluorimeter (SpectraMax Gemini EM Molecular Biomimetics 2023, 8, 599 6 of 16 Devices, San José, CA, USA), scanning electron microscope (Hitachi, S-3400 N, Tokyo, Japan) and a turbomolecular pumped coater (Quorum Technologies, Q150R ES, Lewes, UK) were used.
Modification:Article Title: TCP Doped with Metal Ions Reinforced with Tetragonal and Cubic Zirconia.
Article Snippet: Thus, a cell line of hOB (Cell Applications, Inc., San Diego, CA, USA), t-flasks of 75 cm3 cell culture (Orange Scientific, Braine-l’Alleud, Belgium), Dulbecco’s Modified Eagle Medium/Nutrient F-12 (DMEM-F12, Sigma-Aldrich, Sintra, Portugal), sodium bicarbonate (NaHCO3, Labchem, Laborspirit, Santo Antão do Tojal, Portugal, CAS: 144-55-8), double deionized water (ultrapure water, obtained using an ultrapure water purification system Milli-Q Advantage A10, filtered at 0.22 μm and 18.2 MΩ cm at 25 ◦C), bovine fetal serum (FBS, Biochrom AG, Berlin, Germany, CAS: 9014- 81-7), trypsin (Sigma-Aldrich, Sintra, Portugal, CAS: 9002-07-7), resazurin (Sigma-Aldrich, Sintra, Portugal, CAS: 62758-13-8), spectrofluorimeter (SpectraMax Gemini EM Molecular Biomimetics 2023, 8, 599 6 of 16 Devices, San José, CA, USA), scanning electron microscope (Hitachi, S-3400 N, Tokyo, Japan) and a turbomolecular pumped coater (Quorum Technologies, Q150R ES, Lewes, UK) were used.
Purification:Article Title: TCP Doped with Metal Ions Reinforced with Tetragonal and Cubic Zirconia.
Article Snippet: Thus, a cell line of hOB (Cell Applications, Inc., San Diego, CA, USA), t-flasks of 75 cm3 cell culture (Orange Scientific, Braine-l’Alleud, Belgium), Dulbecco’s Modified Eagle Medium/Nutrient F-12 (DMEM-F12, Sigma-Aldrich, Sintra, Portugal), sodium bicarbonate (NaHCO3, Labchem, Laborspirit, Santo Antão do Tojal, Portugal, CAS: 144-55-8), double deionized water (ultrapure water, obtained using an ultrapure water purification system Milli-Q Advantage A10, filtered at 0.22 μm and 18.2 MΩ cm at 25 ◦C), bovine fetal serum (FBS, Biochrom AG, Berlin, Germany, CAS: 9014- 81-7), trypsin (Sigma-Aldrich, Sintra, Portugal, CAS: 9002-07-7), resazurin (Sigma-Aldrich, Sintra, Portugal, CAS: 62758-13-8), spectrofluorimeter (SpectraMax Gemini EM Molecular Biomimetics 2023, 8, 599 6 of 16 Devices, San José, CA, USA), scanning electron microscope (Hitachi, S-3400 N, Tokyo, Japan) and a turbomolecular pumped coater (Quorum Technologies, Q150R ES, Lewes, UK) were used.
Microscopy:Article Title: TCP Doped with Metal Ions Reinforced with Tetragonal and Cubic Zirconia.
Article Snippet: Thus, a cell line of hOB (Cell Applications, Inc., San Diego, CA, USA), t-flasks of 75 cm3 cell culture (Orange Scientific, Braine-l’Alleud, Belgium), Dulbecco’s Modified Eagle Medium/Nutrient F-12 (DMEM-F12, Sigma-Aldrich, Sintra, Portugal), sodium bicarbonate (NaHCO3, Labchem, Laborspirit, Santo Antão do Tojal, Portugal, CAS: 144-55-8), double deionized water (ultrapure water, obtained using an ultrapure water purification system Milli-Q Advantage A10, filtered at 0.22 μm and 18.2 MΩ cm at 25 ◦C), bovine fetal serum (FBS, Biochrom AG, Berlin, Germany, CAS: 9014- 81-7), trypsin (Sigma-Aldrich, Sintra, Portugal, CAS: 9002-07-7), resazurin (Sigma-Aldrich, Sintra, Portugal, CAS: 62758-13-8), spectrofluorimeter (SpectraMax Gemini EM Molecular Biomimetics 2023, 8, 599 6 of 16 Devices, San José, CA, USA), scanning electron microscope (Hitachi, S-3400 N, Tokyo, Japan) and a turbomolecular pumped coater (Quorum Technologies, Q150R ES, Lewes, UK) were used.
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